All products from TargetMol are for Research Use Only. Rate of FITC-Dextran (4kDa) Flux Across Duodenal Mucosa [ Time Frame: Over 3 hours post FITC-Dextran (4kDa) administration ] This is not a pharmacokinetic or pharmacodynamic measure. IT is produced by introducing cationic (positively charged) diethylaminoethyl groups into selected dextran fractions. fluorescein. Then, add mouse IgE to a concentration of one microgram per milliliter. FITC-Dextrans are polysaccharides comprised of varying lengths of branched glucose molecules and can be used to determine solute, ion and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used. (2) How to make the stocking solution and how is the store conditions? / Dextran-Fluorescein General properties of Biotin label . Gavage the mouse with 150 μl of 80 mg/ml FITC dextran (4kDa). Molecular Probes prepares most of its dextrans by reacting a water-soluble amino dextran with the CAS Number: 60842-46-8 Fluorescein isothiocyanate (FITC)-dextrans are synthesised by coupling fluoresceinyl isothiocyanate (5-isomer) to selected dextran fractions. Do not use stored solutions of FITC in DMSO; prepare fresh on the day of the reaction. FITC dextran is a green fluorescent dextran conjugate with excitation/emission wavelength around 495 nm/515 nm. Of note, passage of 4 kDa FITC-dextran and other paracellular probes such as 51 Cr-EDTA show a linear increase over time . As with morpholinos, caged fluorescein dextran can be injected into the animal cell or the yolk. The content of TRITC substituents ranges from 0.001 to 0.008 per glucose unit. Increased spleen weights generally correlate with the extent of inflammation and anemia. (2) Stocking solution was dissolved in HBSS at a concentration of 100 mg/mL and stored at 4 ℃. The leaf tissue was gently washed to remove surface FITC-dextran . (Catalog #18770) and labeled with Anti-Dextran, Clone DX1, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (open histogram). Surface bound virus fluorescence was quenched using a . 40 kDa. Sections were subsequently directly coverslipped with Dapi mounting media . FITC-dextrans represent a valuable tool to characterize altered blood-brain barrier permeability in animal models. High molecular weight dextran is hard to dissolve. Fluorescein isothiocyanate-dextran (FITC-Dextran 70) is use as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. It's available with the following CF® Dyes: CF®350, CF®488A, CF®633, CF®680, CF®750 and CF®770. Product Identification Product name: FITC-dextran Chemical name: Dextran, (3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H), . All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. Perfuse 12 mL of FITC-dextran solution at the rate of 12 mL per minute. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity. Many ITCs possess promising chemopreventive activities against BC and its recurrence. Vortex at top speed IMMEDIATELY upon adding it to aqueous medium to prevent forming an intractable lump. TdB Labs produce FITC-dextrans from 4 kDa to 2000 kDa. FITC-dextran is supplied as a yellow/orange powder which dissolves freely in water or salt solutions giving a yellow solution. . Buffers at 37 °C were renewed after 20 minutes and again at 30 minutes to remove auto-fluorescent molecules before measurements began. *This assay works best with two or more people - one person does blood collection and the other weighs the microfuge tubes and processes the blood after collection*